Roy Amlot was educated at Dulwich College and called to the Bar in 1963. He practised for 46 years in criminal law, retiring at the end of 2009. He was Treasury Counsel for 14 years and took silk in 1989. He was Chairman of the Bar in 2001 and Treasurer of Lincoln's Inn in 2007. He was head of his chambers for 10 years and served on the Queens Counsel Appointments panel for three years.
He was a governor of Dulwich College for 14 years, is a former Co-Chairman of the Feathers Association and former Trustee of the National Centre for Citizenship and the Law. He is the Chairman of the Earls Court Youth Club and became a Trustee of The Foyle Foundation in 2010.